Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Equisetum via Response Surface Methodology Based on Antioxidant Activity

Flavonoids are one of the most essential compounds in various plants. This study used cellulase-assisted method to extract flavonoid-enriched antioxidants from Equisetum arvense L. (EAL). According a three-factor and three-level central composite design, response surface methodology was obtain maximum total flavonoid yield, thereby providing potent antioxidant activity EAL extracts. The results indicated that optimal condition for extraction 0.52% (base substrate) enzyme concentration enzymolysis 50.58 min at 49.03 °C. rate flavonoids horsetail reached 4.88 mg/g under these conditions. yield 51.23% higher than traditional solid–liquid method. highest DPPH-scavenging ability extracts obtained 0.50% 49.41 46.59 °C 77.36%. HPLC revealed substances had diverse flavonoids. Thus, consistent Therefore, current provided an efficient EAL, facilitating technical support utilizing plant.